A single step purification for recombinant proteins
نویسندگان
چکیده
A 167 base pair DNA cassette has been constructed to facilitate the detection and purification of recombinunt proteins. This cassette, kfc, cncodcs three distinct peptide units: a phosphorylation site for (he CAMP-depcndeni protein base (PKA), called hemptidc, a factor Xa cleavage site, and a calmodulin-binding peptide. Expressed kfc fusio? proteins can be purified from bacterial lysates in one step by allinity chromatography on calmodulin-agurose using EGTA as cluunt. As a test of this system, WC describe the expression. purification and characterization of the PKA binding domain of the microtubule associuted protein (MAP 2).
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